PURExpress(aa, tRNA) Kit from New England Biolabs

Product Specs
ItemPURExpress(aa, tRNA) Kit
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberE6840S
Quantity10 reactions
ApplicationsProteomics & Glycomics
Brand/TrademarkNew England Biolabs Inc (NEB)
Temperature-80 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) MenT nucleotidyltransferase toxins extend tRNA acceptor stems and can be inhibited by asymmetrical antitoxin bindingNat CommunAugust 17, 2023Xibing Xu, Ben Usher, Claude Gutierrez, Roland Barriot, Tom J. Arrowsmith, Xue Han, Peter Redder, Olivier Neyrolles, Tim R. Blower, Pierre Genevauxby Image Lab software (Bio-Rad). To pre-incubate MenT1 with tRNA, a PURExpress Δ(aa, tRNA) kit (NEB, E6840S) was used. 0.33 µl tRNA from the kit was incubated with 0, 2.5, 5, 10 µM MenT1 for 3 h at 37 °C, then

(2) Mechanism of orphan subunit recognition during assembly quality controlCellAugust 3, 2023Yagita Y, Zavodszky E, Peak-Chew SY, Hegde RS.Synthesis Kit,New England Biolabs,Cat#E6800S "PURExpress Δ (aa, tRNA) Kit",New England Biolabs,Cat#E6840S ,, Deposited data,Deposited data,Deposited data ,, Proteomics data,This paper,PRIDE: PXD042773 "Cryo-EM

(3) Expanding the substrate scope of pyrrolysyl-transfer RNA synthetase enzymes to include non-α-amino acids in vitro and in vivoNature ChemistryJuly 1, 2023Riley Fricke, Cameron V. Swenson, Leah Tang Roe, Noah Xue Hamlish, Bhavana Shah, Zhongqi Zhang, Elise Ficaretta, Omer Ad, Sarah Smaga, Christine L. Gee, et al.X-Val-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys (XV-Flag, where X = 7, 13, 14 or 15) was carried out using the PURExpress Δ (aa, tRNA) Kit (NEB, E6840S) according to a previous protocol with slight modifications17. The XV-Flag peptides were produced from

(4) Suppression of Formylation Provides an Alternative Approach to Vacant Codon Creation in Bacterial In Vitro TranslationAngewandte Chemie International EditionDecember 1, 2020Minglong Liu, Vito Thijssen, Seino A. K. JongkeesInformation Chemicals were purchased from Sigma Aldrich, Alfa Aesar and used without further purification. PURExpress (E6840S) was purchased from New England Biolabs. UPLC-MS experiments were performed on an Agilent 6560 Ion Mobility

(5) Ribosome-mediated polymerization of long chain carbon and cyclic amino acids into peptides in vitroNature CommunicationsDecember 1, 2020Joongoo Lee, Kevin J. Schwarz, Do Soon Kim, Jeffrey S. Moore, Michael C. Jewettendogenous tRNAs from being aminoacylated and used in translation. The PURExpressTM Δ (aa, tRNA) kit (NEB, E6840S) was used for polyamide synthesis reaction and the reaction mixtures were incubated at 37 °C for 3 h.

(6) Defects in the Assembly of Ribosomes Selected for β-Amino Acid IncorporationBiochemistryNovember 12, 2019Fred R. Ward, Zoe L. Watson, Omer Ad, Alanna Schepartz, Jamie H. D. CateIn vitro transcription/translation of (fMVF-Flag was carried out using the combination of PureExpress (ΔtRNA, Δaa (E6840S)) and PureExpress (Δribosome (E3313S)) kits (NEB) with the following modifications. To generate the

(7) Fail-safe genetic codes designed to intrinsically contain engineered organismsNucleic Acids ResearchNovember 4, 2019Jonathan Calles, Isaac Justice, Detravious Brinkley, Alexa Garcia, Drew Endywas prepared by supplementing a PURExpress in vitro expression system lacking tRNAs (PURE ΔtRNA, NEB# E6840S). A standard-encoded expression system was built by supplementing PURE ΔtRNA with control tRNAs supplied

(8) Directed Evolution of Glycopeptides Using mRNA DisplayMethods in EnzymologySeptember 23, 2017Satoru Horiya, Jennifer K. Bailey, Isaac J. Kraussincluding commercial BL21 ribosomes, order PURExpress Δ(amino acids, tRNA) as NEB standard catalog item E6840S, and modify this recipe accordingly. Using in-house PURE system: prepare components described in

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